DyLigh 系列荧光标记是一种近年来被广泛应用的新型荧光染料，由于具有很好的光谱宽度、更强的荧光强度，更高的光学耐受性（抗淬灭性）、对pH不敏感、分子较小而渗透性更好的优势，越来越受到科研客户的喜爱。DyLight 种类齐全，如Dylight 350和Dylight405是蓝色荧光，前者替代AMCA，后者可替代Alexa 405；DyLight488是绿色荧光，可完全替代FITC/Cy2/Alexa 488；完全替代Cy3/Rhodamin/Alexa 546/Alexa555的Dylight 549；替代Texas Red/Alexa 594的Dylight 594；Dylight633和Dylight 649（可替代Cy5/Alexa647）则是更强的红色荧光；另外，Dylight还有适配Odyssey系统的近红外和远红外二抗Dylight 680和DyLight800。1（荧光三标图示）：小鼠视网膜组织使用兔源GFAP一抗和DyLight405抗兔IgG（假绿色）标记，同时DyLight 405抗小鼠IgG（假红色）配合小鼠源neurofilament抗体标记神经丝，Cy3驴抗鸡IgG（假蓝色）与鸡源collagenIV抗体标记出蓝色的胶原蛋白IV。以N-没食子酸丙酯-甘油封片，并以配有405nm激光和氪/氩激光的Olympus Flouview1000激光扫描共聚焦显微镜拍摄。2（荧光四标图示）：小鼠视网膜组织使用兔源GFAP一抗和DyLight405抗兔IgG（假绿色）标记，同时DyLight 405抗小鼠IgG（假红色）配合小鼠源neurofilament抗体标记神经丝，Cy3驴抗鸡IgG（假蓝色）与鸡源vimentin抗体标记出蓝色的波形蛋白，DyLight649抗山羊二抗与山羊源collagenIV抗体标记胶原蛋白IV。以N-没食子酸丙酯-甘油封片，并以配有405nm激光和氪/氩激光的Olympus Flouview1000激光扫描共聚焦显微镜拍摄。图 3：使用FACSCalibur流式细胞仪，分析抗-CD3和其他荧光素标记的人外周血淋巴细胞图谱。DyLight 405(绿色)、FITC (黄色)、Cy2(蓝色)标记的二抗都来自Jackson公司。另外Alexa Fluor 488购自Invitrogen。箭头所指处显示AlexaFluor 488在这个实验中有轻微的背景。4：以时间为横坐标，初始荧光强度的百分比为纵坐标绘制的图谱，HEp-2细胞以ANA（Bio-Rad）和各种山羊抗人IgG（H+L）的二抗标记，细胞用不含抗淬灭剂的PBS-90%甘油封片。显示DyLight 405和Alexa488均有较好的光学稳定性，FITC最容易淬灭，Cy2次之。DyLight 405标记的抗体比传统的蓝色CascadeBlue标记的更亮，与Alexa Fluor 405标记的亮度相当，但背景更低；从光稳定性上来说，DyLight 405与Alexa 405均表现优异，均强于CascadeBlue。另外，DyLight荧光标记的二抗比传统的荧光标记以及Alexa都具有更高的性价比。另外，蓝色的DyLight405可以配合绿色DyLight 405、DyLight 549（或DyLight 594）等做出三标荧光实验，更能与DyLight405、DyLight 549（或DyLight 594）、DyLight 649做出四标荧光实验。我们向您推荐美国Abbkine原装进口的液体形式的小包装常用DyLight系列二抗产品，以及Jackson各种规格的全系列DyLight二抗产品。Abbkine是美国专业生产免疫试剂并提供细胞学研究解决方案的公司，其产品主要销往到全球的的大学、科研院所等研究机构。其中Abbkine为您提供最常用的DyLight 405荧光标记二抗产品：DyLight 405 AffiniPure Donkey Anti-Mouse IgG(H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Shp SrProt)Jackson715-475-1500.5mgDyLight 405 AffiniPure Donkey Anti-Mouse IgG(H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Rat, Shp SrProt)Jackson715-475-1510.5mgDyLight 405 AffiniPure Donkey Anti-Mouse IgM, chain specific(min X Hu, Bov, Hrs, Rat Sr Prot)Jackson715-475-1400.5mgDyLight 405 AffiniPure Goat Anti-Mouse IgG(H+L) (min X Hu, Bov, Hrs, Rb, Sw Sr Prot)Jackson115-475-1461.5mgDyLight 405 AffiniPure Goat Anti-Mouse IgG,Light Chain Specific for Western blotting (min X Bov, Gt, Hrs, Hu,Rb, Rat, Shp Ig)Jackson115-475-1740.5mgDyLight 405 AffiniPure Goat Anti-Mouse IgG, Fcγ fragmentspecific (min X Hu, Bov, Hrs Sr Prot)Jackson115-475-0711.5mgDyLight 405 AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 1specific (min X Hu, Bov, Rb Sr Prot)Jackson115-475-2050.5mgDyLight 405 AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2aspecific (min X Hu, Bov, Rb Sr Prot)Jackson115-475-2060.5mgDyLight 405 AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2bspecific (min X Hu, Bov, Rb Sr Prot)Jackson115-475-2070.5mgDyLight 405 AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2cspecific (min X Hu, Bov, Rb Sr Prot)Jackson115-475-2080.5mgDyLight 405 AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 3specific (min X Hu, Bov, Rb Sr Prot)Jackson115-475-2090.5mgDyLight 405 AffiniPure Goat Anti-Mouse IgG (subclasses1+2a+2b+3), Fcγ fragment specific (min X Hu,Bov, Rb Sr Prot)Jackson115-475-1641.0mgDyLight 405 AffiniPure Goat Anti-Mouse IgG, F(ab\')2 fragmentspecific (min X Hu, Bov, Hrs Sr Prot)Jackson115-475-0721.5mgDyLight 405 AffiniPure Goat Anti-Mouse IgM, chainspecific (min X Hu, Bov, Hrs Sr Prot)Jackson115-475-0751.0mgDyLight 405 AffiniPure Rabbit Anti-Mouse IgG, F(ab\')2 fragmentspecific (min X Hu Sr Prot)Jackson315-475-0471.0mgDyLight 405 AffiniPure Sheep Anti-Mouse IgG, Fcγ fragmentspecific (min X Hu, Bov, Hrs Sr Prot)Jackson515-475-0711.5mgDyLight 405 AffiniPure Sheep Anti-Mouse IgG, F(ab\')2 fragmentspecific (min X Hu, Bov, Hrs Sr Prot)Jackson515-475-0721.5mgDyLight 405 AffiniPure Donkey Anti-Rabbit IgG(H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rat, Shp SrProt)Jackson711-475-1520.5mgDyLight 405 AffiniPure IgG Fraction Monoclonal MouseAnti-Rabbit IgG, Light Chain Specific for Western blotting (min XBov, Gt, Ar Hms, Hrs, Hu, Ms, Rat, Shp Ig)Jackson211-472-1710.5mgDyLight 405 AffiniPure Goat Anti-Rabbit IgG, F(ab\')2 fragmentspecific (min X Hu Sr Prot)Jackson111-475-0471.5mgDyLight 405 AffiniPure Polyclonal Mouse Anti-Rabbit IgG (H+L)(min X Hu, Gt, Ms, Shp Sr Prot)Jackson211-475-1091.0mgDyLight 405 AffiniPure Bovine Anti-Goat IgG(H+L) (min X Bov, Ck, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat SrProt)Jackson805-475-1800.5mgDyLight 405 AffiniPure Donkey Anti-Goat IgG (H+L) (min X Ck,GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat Sr Prot)Jackson705-475-1470.5mgDyLight 405 AffiniPure Rabbit Anti-Goat IgG, F(ab\')2 fragmentspecific (min X Hu Sr Prot)Jackson305-475-0471.0mgDyLight 405 AffiniPure Donkey Anti-Rat IgG(H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu,Rb, Shp Sr Prot)Jackson712-475-1500.5mgDyLight 405 AffiniPure Donkey Anti-Rat IgG(H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rb, Shp SrProt)Jackson712-475-1530.5mgDyLight 405 AffiniPure Goat Anti-Rat IgG, Light Chain Specificfor Western blotting (min X Bov, Gt, Hrs, Hu, Ms, Rat, Shp Ig)Jackson112-475-1750.5mgDyLight 405 AffiniPure Goat Anti-Rat IgG, Fcγ fragment specific(min X Hu, Bov, Hrs Sr Prot)Jackson112-475-0711.5mgDyLight 405 AffiniPure Goat Anti-Rat IgG, F(ab\')2 fragmentspecific (min X Hu, Bov, Hrs Sr Prot)Jackson112-475-0721.5mgDyLight 405 AffiniPure Mouse Anti-Rat IgG (H+L) (min X Hu, Bov,Hrs, Ms, Gt, Rb Sr Prot)Jackson212-475-1681.0mgDyLight 405 AffiniPure Mouse Anti-Rat IgG, Fcγ fragmentspecific (min X Hu, Bov, Hrs, Ms Sr Prot)Jackson212-475-1041.0mgDyLight 405 AffiniPure Mouse Anti-Rat IgG, F(ab\')2 fragmentspecific (min X Hu, Bov, Hrs, Ms Sr Prot)Jackson212-475-1061.0mgDyLight 405 AffiniPure Rabbit Anti-Rat IgG, F(ab\')2 fragmentspecific (min X Hu Sr Prot)Jackson312-475-0471.0mgDyLight 405 AffiniPure Donkey Anti-Human IgG(H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Ms, Rb, Rat, Shp SrProt)Jackson709-475-1490.5mgDyLight 405 AffiniPure Donkey Anti-Human IgG,Fcγ fragment specific (min X Bov, Hrs, Ms Sr Prot)Jackson709-475-0981.0mgDyLight 405 AffiniPure Donkey Anti-Human IgM, Fc5 fragmentspecific (min X Bov, Hrs Sr Prot)Jackson709-475-0731.5mgDyLight 405 AffiniPure Goat Anti-Human IgG, Fcγ fragmentspecific (min X Bov, Hrs, Ms Sr Prot)Jackson109-475-0981.0mgDyLight 405 AffiniPure Goat Anti-Human IgG, F(ab\')2 fragmentspecific (min X Bov, Hrs, Ms Sr Prot)Jackson109-475-0971.5mgDyLight 405 AffiniPure Mouse Anti-Human IgG, Fcγ fragmentspecific (min X Bov, Hrs, Ms Sr Prot)Jackson209-475-0981.0mgDyLight 405 AffiniPure Mouse Anti-Human IgG, F(ab\')2 fragmentspecific (min X Bov, Hrs, Ms Sr Prot)Jackson209-475-0971.0mgDyLight 405 AffiniPure Donkey Anti-Sheep IgG(H+L) (min X Ck, GP,Sy Hms,Hrs, Hu,Ms,Rb, Rat Sr Prot)Jackson713-475-1470.5mgDyLight 405 AffiniPure Rabbit Anti-Sheep IgG, F(ab\')2 fragmentspecific (min X Hu Sr Prot)Jackson313-475-0471.0mgDyLight 405 AffiniPure Donkey Anti-ChickenIgY (IgG) (H+L) (min X Bov, Gt, GP, Sy Hms, Hrs,Hu, Ms, Rb, Rat, Shp Sr Prot)Jackson703-475-1550.5mgDyLight 405 AffiniPure Goat Anti-Chicken IgY(IgG) (H+L) (min X Bov, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat, ShpSr Prot)Jackson103-475-1550.5mgDyLight 405 AffiniPure Donkey Anti-Guinea PigIgG (H+L) (min X Bov, Ck, Gt, Sy Hms, Hrs, Hu, Ms, Rb, Rat, Shp SrProt)Jackson706-475-1480.5mgDyLight 405 AffiniPure Goat Anti-ArmenianHamster IgG (H+L) (min X Bov, Hu, Ms, Rb, Rat Sr Prot)Jackson127-475-1600.5mgDyLight 405 AffiniPure Goat Anti-Syrian Hamster IgG (H+L) (minX Bov, Hrs, Hu ,Ms, Rb, Rat Sr Prot)Jackson107-475-1421.0mg